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Image Search Results
Journal: BioTechniques
Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples
doi: 10.2144/btn-2017-0102
Figure Lengend Snippet: Absorbance control values of the samples at 1 cm pathlength measured using a high accuracy dual beam spectrophometer at 22°C and 0.8 nm spectral bandwidth. The values in the parentheses are CV (%) of the mean from N = 9 (genomic DNA) and N = 4 (oligoncleotide samples), except for the *SRM 2082 components that are the standard uncertainties calculated as described in the NIST certificate of analysis ( https://www.nist.gov/srm ) calcuated using extensive measurements.
Article Snippet:
Techniques: Control
Journal: BioTechniques
Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples
doi: 10.2144/btn-2017-0102
Figure Lengend Snippet: Percentage of control values and coefficients of variation (% CV) using microvolume spectrophometers (MV Spec), and a microvolume (MV) plate reader. The values are the average of data acquired over three days as shown in , with the exception of MV Spec C, where the data were acquired in one day. The values in the parentheses are 1 CV (%) and the number (N) of measurements.
Article Snippet:
Techniques: Control
Journal: BioTechniques
Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples
doi: 10.2144/btn-2017-0102
Figure Lengend Snippet: Mass concentration values of human genomic DNA samples obtained using the different methods. Absorbance values and derived mass values of the human genomic DNA samples from a double-beam (DB) spectrophotometer, microliter volume (MV) spectrophotometers, microvolume (MV) plate reader, fluorescent dye binding, and digital PCR assays.
Article Snippet:
Techniques: Concentration Assay, Derivative Assay, Spectrophotometry, Binding Assay, Digital PCR, Lambda DNA Preparation
Journal: BioTechniques
Article Title: Limitations of methods for measuring the concentration of human genomic DNA and oligonucleotide samples
doi: 10.2144/btn-2017-0102
Figure Lengend Snippet: Mass concentration of the human genomic DNA sample calculated from droplet digital PCR data with different assumptions of the droplet size.
Article Snippet:
Techniques: Concentration Assay, Digital PCR